Super-intensive L. vannamei cultivation produces waste that causes pollution, while simultaneously an appropriate water source, meeting water quality standards, is required for its sustainability. Hence, aquaculture waste poses a significant challenge in minimizing the source of water pollution. Therefore, this study aimed to determine the physicochemical and biological properties of the quality of water source, used for the super-intensive L. vannamei farming. The experiment was conducted at 18 points of the water source of super-intensive L. vannamei cultivation. Samples were categorized into three groups: vertical at distances of 0, 100, and 200 meters, while horizontal consisted of 6 points. The study found 21 plankton genera comprising 13 phytoplankton and 8 zooplankton with low diversity (H') and stability. Plankton uniformity (E) below 0.4 ≤ E ≤ 0.6 indicates low uniformity, and no dominant individuals (D) were detected. The temperature, pH, TSS, and BOT correlation showed a moderate relationship. The variables NO3, NO2, PO4, and DO were in the low category, while salinity, NH4, and NH3 showed a very low correlation. In terms of distance, a significant difference (P>0.05) was detected in water quality based on Permen KP. No. 75 of 2016. Thus, the seawater quality standards for superintensive vannamei shrimp ponds are in appropriate conditions, and the seawater quality in front of the super-intensive pond as a quality standard is suitable for cultivation